Download e-book for iPad: Advances in Lipid Methodology. Volume 4 by W. W. Christie (Auth.)

By W. W. Christie (Auth.)

ISBN-10: 0857097997

ISBN-13: 9780857097996

ISBN-10: 0951417177

ISBN-13: 9780951417171

This is often the fourth quantity of an occasional sequence of evaluation volumes facing points of lipid technique. As with the 1st 3 volumes, subject matters were chosen which have been constructing speedily in recent times and feature a few value to lipid research. The authors are all prime foreign experts.

Topics coated contain: research of plant lipoxygenase metabolites, preparative high-performance liquid chromatography of lipids, structural research of fatty acids, and research of strong isotopes in lipids, between others

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Additional resources for Advances in Lipid Methodology. Volume 4

Example text

Peak identification has been by comparison with standards, trapping and subsequent identification of component fatty acids by GLC, by retention time relative to a known component and by liquid chromatography-mass spectrometry. 1. Theory The resolution of molecular species by HPLC depends on the fatty acyl chains 48 SEPARATIONS OF MOLECULAR SPECIES OF PHOSPHOLIPIDS of the lipid interacting with the alkyl ligand forming the hydrophobic matrix of the stationary phase. Elution is then effected using polar solvents, usually comprising mixtures of acetonitrile or methanol, and propan-2-ol or water.

21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 58. , Biochim Biophys. Acta, 1001, 274-281 (1989). W. , Lipids, S, 678-683 (1970). Galliard,T. , 124, 431-438 (1971 ). -S. , Lipids, 12, 324-326 (1977). R. , J. Biol Chem, 261, 1099-1104 (1986). D. , in Lipoxygenase and Lipoxygenase Pathway Enzymes, pp. J. Piazza, Am. Oil Chem. Soc. Press, Champaign, IL). D. , Biochim Biophys. Acta, 1124, 59-65 (1992).

Arch. , 283, 409-416 (1990). Hamberg,M. , Plant Physiol, 99, 987-995 (1992). Blee,E. , Biochem. Biophys. Res. , 173, 1354-1360 (1990). Blee,E. , Arch. , 2S4, 43-52 (1987). Hamberg,M. , 110, 807-815 (1996). Blee,E. , 4, 113-123 (1993). , SO, 219-224 (1996). A. , Biochim. Biophys. Acta, 877, 447-457 (1986). , Lipids, 24, 249-255 (1989). , Chem Phys. Lipids, 43, 55-67 ( 1987). , Hamberg,M. , J. Biol. , 267, 14738-14745 (1992). Hamberg,M. ,Arch. , 30S, 115-122 (1993). , Free Radie. , 7, 65-86 (1989).

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Advances in Lipid Methodology. Volume 4 by W. W. Christie (Auth.)


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